By Nord F F (ed)
Advances in Enzymology and comparable parts of Molecular Biology is a seminal sequence within the box of biochemistry, delivering researchers entry to authoritative stories of the newest discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, offering an unmatched view of the old improvement of enzymology. The sequence deals researchers the newest figuring out of enzymes, their mechanisms, reactions and evolution, roles in advanced organic approach, and their software in either the laboratory and undefined. every one quantity within the sequence good points contributions through prime pioneers and investigators within the box from worldwide. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.
With its wide selection of subject matters and lengthy old pedigree, Advances in Enzymology and comparable components of Molecular Biology can be utilized not just by way of scholars and researchers in molecular biology, biochemistry, and enzymology, but additionally by means of any scientist drawn to the invention of an enzyme, its homes, and its purposes.
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Additional info for Advances in Enzymology and Related Areas of Molecular Biology, Volume 30
Although DCMU was found to inhibit cyt f reduction and noncyclic phosphorylation, the absorbancy changes a t 363 mp were affected to only a small extent. Antimycin A, an inhibitor of electron transport a t the cyt b site in mitochondria, inhibited noncyclic photophosphorylation and the reduction of cyt f by red light, but the reactions of cyt b6 were virtually unaffected. From these experiments, it would appear unlikely that cyt b6 is an electron carrier on the main electron transport pathway between the pigment systems.
These fluorescence experiments support the conclusion derived from the 02-yield experiments in long flashes. After illumination, photosystem 2 contains a pool of reductants, 1 equiv/40 chl, which is tenfold higher than the individual concentrations of the photosystem 1 electron carriers, cyt f, plastocyanin, and P-700, and of the primary catalyst of photosystem 2 (Q). + + + + PHOTOCHEMICAL SYSTEMS O F PH3TOSYNTHESIS 29 Kok (151) altered the redox potential of a chloroplast suspension with ferroxyanide-ferricyanide mixtures, and from the variation of the initial fluorescence with redox, he estimated an Eo’ of approximately +I80 mV for the primary reductant of photosystem 2.
PARTICLES OBTAINED BY T H E DIGITONIN PROCEDURE I . 5% digitonin for 30 min a t 0°C. The chlorophyll-containing particles were separated by differential centrifugation a t the following speeds : 1OOOg for 10 min, 10,OOOg for 30 min, 50,OOOg for 30 min, and 144,OOOg for 60 min. The pellets from the centrifugations were resuspended in phosphate buffer and designated lOOOg, lO,OOOg, 5O,OOOg, and 144,0009 fractions. The final supernatant was designated 144,0009 supernatant or simply S. 76 fractions.
Advances in Enzymology and Related Areas of Molecular Biology, Volume 30 by Nord F F (ed)